T4 phage β-glucosyltransferase t4-bgt
WebProduct name T4 Phage β-glucosyltransferase (T4-BGT) Other means of identification Product No M0357 Synonyms None Recommended use of the chemical and restrictions … WebSep 1, 1999 · beta-Glucosyltransferase (BGT) is a DNA-modifying enzyme encoded by bacteriophage T4 which catalyses the transfer of glucose (Glc) from uridine …
T4 phage β-glucosyltransferase t4-bgt
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WebAug 22, 2024 · Therefore, a second enzyme, T4 Phage β-glucosyltransferase (T4-BGT), is used to catalyze the transfer of a glucose moiety from uridine diphosphate glucose (UDP-Glc) to 5hmC resulting in the production of 5- (β-glucosyloxymethyl)cytosine (5gmC) (Fig. 1b ), which is detected as a new CE peak resolved from that of 5mC (Fig. 2 ). 5fC can be …
WebJul 4, 2014 · To further explore 5hmC localization in the oocyte genome, we carried out a 5hmC detection assay using T4 phage β-glucosyltransferase (T4-BGT, which distinguishes between 5hmC and 5mC because glucosylated 5hmC is indigestible by MspI) to detect 5hmC level in specific repetitive sequences: intracisternal A particle (IAP), murine … WebAug 17, 2001 · Glycosyltransferases have been classified into 49 different families based on both sequence similarity and substrate/product stereochemistry 2, 3, 4. To date, only eight crystal structures are available and one of them is the unclassified β-glucosyltransferase (BGT) of the Escherichia coli T4 bacteriophage 5, 6, 7, 8, 9.
WebJul 30, 1979 · T4 phage β-glucosyltransferase: Substrate binding and proposed catalytic mechanism 1999, Journal of Molecular Biology Show abstract Crystallization and preliminary X-ray studies of T4 phage β-glucosyltransferase 1988, Journal of Molecular Biology Show abstract Initiation of transcription at phage T4 late promoters with purified … WebT4 Phage β-glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double …
WebEdward B Goldberg works at Department of Molecular Biology and Microbiology, and is well known for Phage T4, Tail Fibers and Viral Escherichia. download . FREE KOL List . KOL …
WebOct 22, 2004 · Bacteriophage T4 α-Glucosyltransferase DNA modification Mutagenesis In contrast to the DNA of most life forms, the DNA of bacteriophage T4 contains hydroxymethylated cytosine (HMC) instead of unmodified cytosine. The HMC nucleotides are further modified by the presence of glucose covalently linked to the 5-hydroxymethyl … sheridan radiology svcs of west floridaWebM0357 - T4 Phage β-glucosyltransferase (T4-BGT) Revision date 12-Apr-2024 4. First-aid measures Description of first aid measures Inhalation Remove to fresh air. Eye contact … sheridan radiology services of west flWebIn molecular biology, Bacteriophage T4 beta-glucosyltransferase refers to a protein domain found in a virus of Escherichia coli named bacteriophage T4. Members of this family are … sheridan ramseyWebT4 Phage β Glucosyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, … spt sd-9263w dishwasherWebusing T4 β-glucosyltransferase (T4-BGT). They then pulled down the 5-gmC with another protein, J-binding protein 1 (JBP1), which has a strong affinity for 5-gmC. The pulled-down material can be used in quantitative PCR, microarrays, and sequencing. An … spt season ticketWebT4 Phage β-glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double … sheridan railWebNov 1, 2004 · T4 Phage β-Glucosyltransferase: Substrate Binding and Proposed Catalytic Mechanism. Article. ... T4 phage beta-glucosyltransferase (BGT) is an inverting glycosyltransferase (GT) that transfers ... sptservice-00021