WebApr 30, 2024 · Seahorse XFe96 Extracellular Flux Analyzer. 2. CO 2 incubator (set to 37 °C, 5% CO 2, 95% humidity). 3. NonCO 2 incubator (set to 37 °C). 4. pH meter. 5. Tissue lyser (Qiagen). 6. API-4000 triple quadrupole mass spectrometer (AB Sciex) coupled with a HPLC system (Agilent) and CTC PAL HTS autosampler (PAL System). 3 Methods WebApr 14, 2024 · Assay were performed using the Seahorse XFe96 analyzer (Seahorse Bioscience, Agilent) according to the manufacturer’s instructions (Seahorse XF Glycolysis Stress Test Kit#103020-100).
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WebThe .gov means it’s official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site. WebThe XFe-24 flux analyzer measures real time kinetic of the media flux in live cellular environment to determine in vitro oxygen consumption rate (OCR), and extracellular acidification rate (ECAR), in order to assess cellular metabolic functions such as oxidative phosphorylation, glycolysis, and fatty acid oxidation. XFe-24 assays apply to all fields of … shannon brooks state farm amarillo
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