Cytofectin
Webcytofectin, (i) efficiently transfects ODNs and plasmids into many cell types in the presence or absence of 10% serum in the medium, (ii) uses a 4- to 10-fold lower concentration of … WebCytofectin™ - Efficient Transfection of Antisense Oligonucleotides To improve oligonucleotide delivery, researchers at Gilead Sciences developed the Cytofectin™ GS …
Cytofectin
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Webwe introduced Cytofectin GSV, a transfection reagent, designed at and licensed by Gilead, to be used to transport antisense oligonucleotides into cells even in the presence of … WebNational Center for Biotechnology Information
GS3815 cytofectin (Glen Research, Sterling, VA) is a formulation of two equivalents of a cationic lipid (dimyristylamidoglycyl- N -α-isopropoxycarbonyl-arginine dihydrochloride, GS3815) with one equivalent of the zwitterion l -α-dioleoylphosphatidylethanolamine (DOPE, Avanti Polar Lipids). WebApr 4, 2002 · Cytofectin components Synthesis of the ( ± )-N- (3-aminopropyl)-N,N-dimethyl-2,3-bis (alkyloxy)-1-propanaminium bromides. Racemic 1-dimethylamino-2,3-propanediol (0.96 g) (Janssen Chimica, Geel, Belgium) was converted to the disodium salt in situ by treatment with sodium hydride (60% in oil, 0.8 g) in tetrahydrofuran (70 ml).
WebAlthough optimal for the transfection of antisense oligonucleotides, Cytofectin GSV has also been found to be suitable for plasmid transfection. In December 1998, Gilead's antisense technology, including the patents covering the C-5 Propynes and Cytofectin GSV, was purchased by Isis Pharmaceuticals, Inc. WebThe large number of cytofectin and co-lipid combinations currently used for lipoplex-mediated gene delivery reflects the fact that the optimal cytofectin/co-lipid combination varies with the...
WebSep 17, 1996 · A cytofectin composition comprising an amine having bonded to an attached carbon chain at least a pair of same or different lipoyl moieties selected from a group consisting of an alkyl, alkenyl, alkynyl, alkanoyl, alkenoyl, or alkynoyl groups and at least two hydroxylated, ether containing, or acyloxy containing alkyl, alkenyl, or alkynyl …
WebFeb 5, 1999 · A 1/3 (w/w) ratio of DNA/liposome (1/1 nucleotide/ cytofectin mol ratio) was determined to be optimal for transfection. In addition, we found that the transfection complexes were able to penetrate ... sachs creditWebJan 1, 2013 · After thawing, the cytofectin aliquot can be kept at 4 °C for several weeks. 3 Methods. Carry out all procedures at room temperature unless otherwise specified. 3.1 Pollen Tube Cultivation and AODN Treatment. For simple analysis of pollen tube growth parameters, optimal volume per sample is 200 μL of final pollen tube culture. ... is hope and expectation the same thingsachs covered bridge gettysburg hauntedWebAS-ODN with cytofectin compared with the corresponding S-ODN with cytofectin (530 190 lm, n = 3, >87 PTs per replicate; Figure S2). The pollen germination rate was not significantly reduced by the AS-ODN with cytofectin (59 5%, n = 3, >310 PTs per experiment) compared with the corresponding S-ODN with cytofectin (64 10%, n = 3, sachs covered bridge storyWebAug 1, 1996 · To prepare the cytofectin/ON complexes for transfection in one well of a 6-well tissue culture plate, the ON was first diluted into 100 µl pre-warmed Opti-MEM (Gibco BRL) in polystyrene plastic. Similarly, the cytofectin was diluted in another container ( 5 ). is hope an original vampireWebKeywords: Cholesterol, cytofectin, gene delivery, liposomes, monovalent, review. Abstract: A large number of liposome-based non-viral gene and siRNA delivery systems include … is hope an originalWebApr 1, 2001 · Cytofectin formulations have also been optimized for vaccination applications. Vaxfectin, a new cytofectin formulation, has recently shown significant enhancement of humoral immune responses against several antigens compared to naked pDNA ( 19 ). sachs covered bridge address