WebNov 25, 2024 · Samples from 30 high-sensitive and 30 resistant lines were collected and bulked for each extreme for resequencing and BSA (bulk segregant analysis). Pathogenicity tests using colonized sorghum grains as inoculum White sorghum grains, soaked overnight in a 250 ml flask, were used for inoculum preparation. WebQuantitative trait locus (QTL) mapping and bulk segregant analysis using a set of WGS data (QTL-seq) detected a major QTL on the upper arm of chromosome 3 for BPB, SB, and DTH traits within the 1.0–1.9 Mb position. Additional QTLs associated with BPB and SB were also identified from other chromosomes by the QTL-seq analysis. The QTLs ...
Bulked segregant analysis - Wikipedia
WebFeb 13, 2024 · This method combined a high-throughput whole-genome re-sequencing with a bulked-segregant analysis, which represents a quicker and more efficient method of identifying a target gene. WebMar 18, 2024 · Generally, F2 progeny is sufficient for segregant bulk construction and analysis, therefore the BSA method is often considered as rapid and efficient compared to quantitative trait locus (QTL ... truck stop raphine va
Chapter 4
WebDec 21, 2024 · INTRODUCTION. Bulk segregation analysis (BSA) was first proposed by Michelmore et al. () (Department of Vegetable Crops, University of California, Davis) and was used to identify molecular markers linked to DM5/8 (downy mildew resistance loci in lettuce) in F 2 populations.Sample pooling was performed according to the two extreme … WebBulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative … WebFeb 7, 2024 · Bulk segregant analysis is a technique for identifying the genetic loci that underlie phenotypic trait differences. The basic approach is to compare two pools of individuals from the opposing tails of the phenotypic … truck stop rmis